Morphological analysis, agronomic characteristics, species identification, and phylogenetic analysis of Pseuderanthemum sp. using the rbcLgene
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Published:2024-03-27
Issue:
Volume:
Page:38-44
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ISSN:2672-7277
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Container-title:Asia Pacific Journal of Molecular Biology and Biotechnology
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language:en
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Short-container-title:APJMBB
Author:
Duong Thieu Van1, Mai Do Van2, Trong Pham Thanh1, Nam Tran Van Be3, Hung Mai Van4
Affiliation:
1. Faculty of Pharmacy & Nursing, Tay Do University, Can Tho, Vietnam 2. Faculty of Pharmacy, Nam Can Tho University, Can Tho, Vietnam 3. Institute of Food and Biotechnology, Can Tho University, Can Tho, Vietnam 4. Department of Natural Sciences, VNU University of Education, Hanoi, Vietnam
Abstract
The study aimed to evaluate the agronomic characteristics of eleven samples of Pseuderanthemum sp. variety based on the results of the morphological survey on stem height, root length, leaves, combining molecular biology methods to determine their genetic relationships by building a phylogenetic tree based on the plastid gene rbcL sequence. Initial results of an evaluation of the genetic diversity of eleven Pseuderanthemum sp. samples showed that there were differences in stem length, leaf width to root length. Based on the analysis results from the pedigree tree, the tree samples with similar survival indexes and genetically close to each other were classified into two main groups. Based on molecular data, eleven varieties of Pseuderanthemum sp. in the study have been identified, all belonging to the species Pseuderanthemum sp. have a high similarity coefficient above 98% and 100% coverage. Although the geographical location of the samples is variable, it is necessary to investigate other gene sequences related to morphological and biochemical characteristics, such as bioactive compounds in leaves and fruits to have more accurate conclusions about this species.
Publisher
Malaysian Society for Molecular Biology and Biotechnology
Reference18 articles.
1. Chase, M. W., Cowan, R. S., Hollingsworth, P. M., Berg, C. V. D., Madrinans, S., Petersen, G., Seberg, O., Jørgsensen, T., Cameron, K. M., Carine, M., Pedersen, N., Hedderson, T. A. J., Conrad, F., Salazar, G. A., Richardson, J. E., Hollingsworth, M. L., Barraclough, T. G., Kelly, L., & Wilkinson, M. 2007. A proposal for a standardised protocol to barcode all ADN plants. Taxonomy Journal 56(2): 295-299. 2. Chen, S., Yao, H., Han, J., Liu, C., Song, J., Shi, L., Zhu, Y., Ma, X., Gao, T., Pang, X., Luo, K., Li, Y., Li, X., Jia, X., Lin, Y., & Leon, C. 2010. Validation of the ITS2 region as a novel DNA barcode for identifying medicinal plant species. PLoS ONE 5 (1): e8613. 3. Chiou, S. J., Yen, J. H., Fang, C. L., Chen, H. L., & Lin, T. Y. 2007. Authentication of medicinal herbs using PCR-amplified ITS2 with specific primers. Planta media 73 (13): 1421 – 1426. 4. Dieu, H. K., Loc, C. B., YaMaSaKi, S., & HiRaTa, Y. 2005. The ethnobotanical and botanical study on Pseuderanthemum palatiferum as a new medicinal plant in the Mekong Delta of Vietnam. Japan Agricultural Research Quarterly (JARQ) 39(3): 191- 196. 5. Doyle. J.L., and Doyle J.J.,.1990. Isolation of plant DNA from fresht issue. Focus (12): 13-15.
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