Affiliation:
1. National Research Center for Hematology
2. Crimean Republican Blood Centre
3. Orenburg Regional Blood Bank
4. Mordovian Republican Blood Transfusion Centre
5. Sakha (Yakutia) Republican Blood Transfusion Centre
Abstract
Introduction. Occult hepatitis B virus (HBV) revelation in HBV nuclear antigen testing is of particular importance to prevent transfusion infection.Aim — the identification of factors affecting the anti-HBc detection rate in donated transfusable blood components from different regions of Russia.Materials and methods. A cohort screening single-stage epidemiological study was conducted with 2,000 donor blood samples, 500 samples per each of four regions of the country, the Republics of Crimea (Simferopol) and Sakha (Yakutia), the cities of Saransk and Orenburg. Data on 968 blood samples from the National Research Center for Hematology’s donor bank were used as reference. The testing targeted HBV nuclear antigen antibodies. Positive donated blood samples were additionally tested for IgM and virus surface antigen antibodies using Abbott and Vector-Best commercial reagent kits.Results. Donor demographic profiles differed insignificantly across members of the Russian Federation. Males predominated among the donors (69.6 %). Anti-HBc was detected in 219 of 2,000 samples examined (10.9 %). The donor blood sample anti-HBc detection rate ranged from 6.0 to 21.6 %, depending on the region. Anti-HBc-positive proportions in Orenburg, Crimea, Mordovia and Sakha comprised 8.2, 8.0, 6.0 and 21.6 %, respectively (p < 0.01). First-time donors had anti-HBc in 8.06, regular donors — in 11.29 % cases. The anti-HBc detection rate varied with donor’s age, being zero or near 1 % in 20-yo or younger people. Acute HBV antibodies had zero rate in Orenburg at zero or low-titre (< 100 mIU/mL) protective antibodies; 31 total samples, 15 low-titre and 16 negative for protective antibodies. In Simferopol, acute phase antibodies were negative in 7 blood samples containing high-titre protective antibodies (> 100 mIU/mL) and in 5.0 % samples with their low or zero levels. In Yakutian donors, acute phase antibodies were revealed only at protective antibodies negative. In Saransk, this marker was equal-proportion at zero and high-titre protective antibodies (3.3 % each).Conclusion. Transfusion component procurement from younger donors should be prioritised as enhancing haemotransfusion viral safety. Positive occult HBV tests were less common in regions with low HBV incidence.
Publisher
National Medical Research Center of Hematology of the Ministry of Health of the Russian Federation
Reference18 articles.
1. Infectious incidence in the Russian Federation for January–December 2018. Data on infectious and parasitic diseases for January–December 2018. Electronic resource https://rospotrebnadzor.ru/activities/statistical-materials/statictic_details.php?ELEMENT_ID=11277 (In Russian).
2. Tandoi F., Caviglia G.P., Pittaluga F., et al. Prediction of occult hepatitis B virus infection in liver transplant donors through hepatitis B virus blood markers. Dig Liver Dis. 2014; 46(11): 1020–1024. DOI: 10.1016/j.dld.2014.07.172.
3. Caviglia G.P., Abate M.L., Tandoi F., et al. Quantitation of HBV cccDNA in anti-HBc-positive liver donors by droplet digital PCR: A new tool to detect occult infection. J Hepatol. 2018; 69(2): 301–307. DOI: 10.1016/j.jhep.2018.03.021.
4. Raimondo G., Navarra G., Mondello S., et al. Occult hepatitis B virus in liver tissue of individuals without hepatic disease. J Hepatol. 2008; 48(5): 743–746. DOI: 10.1016/j.jhep.2008.01.023.
5. Seed C.R., Maloney R., Kiely P., Bell B., Keller A.J., Pink J., Blood Service Medical Services Lookback Team. Infectivity of blood components from donors with occult hepatitis B infection — results from an Australian lookback programme. Vox Sang. 2015; 108 (2): 113–122. DOI: 10.1111/vox.12198.