POTENCY OF YEAST AS A BIOCONTROL AGENT OF OCHRATOXIN A-PRODUCING FUNGI AND ITS EFFECT ON ARABICA COFFEE TASTE

Author:

Dharmaputra Okky S.,Retnowati Ina,Nurfadila Nijma

Abstract

Biocontrol agents can be used to control mycotoxigenic fungi, which include different species of yeast. The objectives of this research were to select yeast isolates that can inhibit the growth of ochratoxin A (OTA)-producing fungi (Aspergillus ochraceus BIO 37310) and to increase the taste of Arabica coffee processed using wet and semi-wet methods. Twenty-two yeast isolates (KA, KA2, KB, KB2, KC, KD, Endomyces decipiens BIO 131215, E. fibuliger BIO 132216, BIO 132217, BIO 13218, BIO 132219, BIO 132220, Candida krusei (= Issatchenkia orientalis) BIO 211285, BIO 211286, BIO 211287, BIO 211288, BIO 211289, BIO 211290, BIO 211291, Saccharomyces cerevisiae BIO 341363, BIO 341364, and BIO 341365) were screened for their antagonistic property against A. ochraceus BIO 37310 in vitro using well (dip) test method. The results showed that C. krusei (BIO 211287, BIO 211288, and BIO 211289) inhibited A. ochraceus BIO 37310. In vivo the highest yeast population was found in coffee beans processed using a semi-wet method inoculated with C. krusei BIO 211288 (46,222 ± 9,576 cfu/g), which was not significantly different from that of the coffee beans inoculated with C. krusei BIO 211287 (36,333 ± 14,000 cfu/g). The three yeast isolates were also able to grow either in coffee beans processed using wet or semi-wet methods inoculated with A. ochraceus BIO 37310 and each yeast isolate. Interaction between the three yeast isolates and A. ochraceus BIO 37310 resulted in E-type interaction, i.e. the fungus was not able to grow anymore, while the yeasts grew further. The total cupping scores of coffee beans inoculated with the three yeast isolates were higher than those of coffee beans uninoculated and inoculated with commercial lactic acid bacteria. The three yeast isolates could be used as biocontrol agents of A. ochraceus BIO 37310 and increase the sensorial quality of coffee beverages.

Publisher

Seameo Biotrop

Subject

General Agricultural and Biological Sciences,Ecology,Biotechnology

Reference27 articles.

1. Atmawinata O. 2002. Peranan uji citarasa dalam pengendalian mutu kopi. Materi Pelatihan Uji Citarasa Kopi. Pusat Penelitian Kopi dan Kakao, Jember. [The role of cupping test in controlling the quality of coffee beans. (Paper presented at Training on Coffee Cupping Test at the Indonesian Coffee and Cacao Research Institute, Jember.

2. BPOM [Badan Pengawas Obat dan Makanan Republik Indonesia]. 2018. Peraturan Badan Pengawas Obat dan Makanan Republik Indonesia Nomor 8 Tahun 2018 tentang Batas Maksimum Cemaran Kimia dalam Pangan Olahan. Jakarta (ID): Badan POM RI.

3. Brazil. 2011. Rules on maximum permitted limits for mycotoxins in foods and beverages. Resolution RDC No. 7 of February 18, 2011. Brasilia (BR): Brazilian Ministry of Health.

4. Bui-Klimke TR, Wu F. 2015. Ochratoxin A and human health risk: A review of the evidence. Crit Rev Food Sci Nutr 55(13):1860-9. DOI: 10.1080/10408398.2012.724480

5. Clark HA, Snedeker SM. 2006. Ochratoxin A: Its cancer risk and potential for exposure. J Toxicol Environ Health B 9:265-96.

Cited by 1 articles. 订阅此论文施引文献 订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3