In Vitro Analysis of Genotoxic Effects of Dental Panoramic Radiography: Evidence of Potential Genotoxicity Using the Comet Test-Reference-Cited by-同舟云学术

In Vitro Analysis of Genotoxic Effects of Dental Panoramic Radiography: Evidence of Potential Genotoxicity Using the Comet Test

Published:2023-01-04 Issue:1 Volume:17 Page:e03108
ISSN:1981-982X
Container-title:Revista de Gestão Social e Ambiental
language:
Short-container-title:RGSA

Author:

de Oliveira Welison Abreu^ORCID,Vasconcelos Carolina Pinheiro^ORCID,Sagica Fernanda do Espirito Santo^ORCID,De Oliveira Edivaldo Herculano Correa^ORCID

Abstract

Objective: To test the genotoxic potential of panoramic radiography using the comet test. Methods: An in vitro cell line derived from the human periodontal ligament was subjected to two exposure times - 0.7 and 2.5 seconds. The data obtained were analyzed using the KOMET 6® program. Results and Conclusion: Although no significant differences were observed in cells exposed for 2.5 seconds in relation to the control, the exposure interval of 0.7 seconds produced a statistically higher amount of DNA damage than in the control. Thus, we confirm the potential genotoxicity of panoramic radiography. The presence of greater amount of DNA damage at the shortest exposure interval can be explained by the fact that the damage levels must reach a threshold to activate the DNA repair system, which would have happened at the longer exposure interval. Originality/Value: Previous studies were based only on the micronucleus assay and presented conflicting results. The comet test can detect both single-strand and double-strand breaks.

Publisher

RGSA- Revista de Gestao Social e Ambiental

Subject

Management, Monitoring, Policy and Law,Geography, Planning and Development

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