Analysis of Tissue Repair After Application of Calcium Hydroxyapatite in Wistar Rats

Abstract

Objective: The objective of this study was to evaluate the effects of Calcium Hydroxyapatite (HaCa) on tissue repair process in Wistar rats.   Theoretical Framework: Collagen is a fundamental element produced by the extracellular matrix during the wound healing process. In the beauty market, there are collagen biostimulators based on HaCa.   Method: Wounds were created in the dorsal region of 16 female rats, divided into 2 groups with 8 animals each. In the case group, 0.2ml of HaCa was injected around the wounds, while the control group received no product. The animals were analyzed for 30 days. During this period, the wounds and their characteristics during the tissue repair phases were observed both macroscopically and histologically.   Results and Discussion: The reduction of wounds in both the case and control groups was greater between the 5th and 10th day. There was no significant difference between the groups. Microscopically, the skin fragment removed for analysis showed the same characteristics in both groups, with complete re-epithelialization, deposition of collagen fibers, and no signs of exacerbated inflammation.   Research Implications: It was observed that HaCa does not interfere with the tissue repair process, without causing adverse reactions such as inflammation, showing that the collagen biostimulator is a biocompatible material.   Originality/Value: This was the first study to investigate the application of a collagen biostimulator in the tissue repair process, demonstrating that HaCa is harmless to the animals' cutaneous tissues and presents good durability when injected into the subcutaneous tissue.