Diversity of Decomposer Bacteria in Eco Enzyme Fermentation Process of Organic Materials Using Oxford Nanopore Technology (ONT) and its Effectiveness in Inhibiting E-coli in Fish Pond with Water Mineral Soil

Abstract

Objective: This study aims to determine the characteristics of eco enzymes in the form of aroma, color, pH eco enzymes and identify the diversity of bacteria that play a role during the fermentation process.   Method/design/approach: The research was carried out from August to December 2022, the first stage of making eco enzymes fermentation 3 months of organic waste three variants of Citrus sinensis Osb peel, Ananas comosus peel, and Citrullus lanatus peel, combined with 2 variants of vegetable residue of fern plants; Stenochlaena palutris and Diplazium esculentum. Eco enzyme yields were organoleptic tested and measured bacterial diversity with the Oxford Nanopore Technology (ONT) technique.   Results and conclusion: Eco enzyme harvest organoleptic test results: pH 3.74, ethanol content 2.41%, color 1502 Pt.Co and the characteristic smell of fermentation. Bacterial diversity Test of bacterial diversity that plays a role during the eco enzyme fermentation process was carried out using the Oxford Nanopore Technology (ONT) technique, identified the diversity of lactic acid bacteria (BAL) at the taxon level, Phylum 7, Order 19, Familia 37, Genus 98 and species 269 lactic acid batteries (BAL). Eco enzyme 1 liter in 1000-1 liter of mineral groundwater fish pond can inhibit E-coli 36.1% of the original population.   Research implications: This research contributes to the body of knowledge on eco enzymes, bacterial diversity in fermentation processes, and their potential applications in waste management and antibacterial activities. The implications of this study can pave the way for further research, innovations, and practical implementations in the field of eco-friendly technologies and sustainable waste management practices   Originality/Value: The originality of this research lies in its focus on eco enzymes and bacterial diversity, while the value lies in its contribution to waste management practices, antibacterial applications, and advancements in environmentally friendly technology.