Influence of medium type and culture method on the multiplication efficiency of Chlorophytum comosum (Thunb.) Jacques in in vitro conditions

Abstract

In response to the challenges posed by modern plant micropropagation techniques, a promising technology for growing shoots temporary immersed in nutrient solution (temporary immersion system, TIS) using SETIS™ bioreactors has been developed. In this experiment, the suitability of this technology for the propagation of Chlorophytum comosum (Thunb.) Jacques was assessed. In vitro culture was carried out using a conventional technique on solid media and liquid media using the SETIS™ bioreactor. In addition, two culture media differing in macro- and micronutrient content (Murashige & Skoog and Rugini OM), while having the same set of phytohormones were evaluated in both systems. Explants obtained from the flower stalk of the plants were used to establish the culture. The effectiveness of the cultures after the first and second subculture was assessed. The study has demonstrated that the efficiency of liquid culture carried out using the SETIS™ bioreactor is higher compared to the conventional culture. The highest multiplication coefficient, fresh weight of regenerants and RGR index value in bioreactor cultures was recorded on Rugini OM medium. No statistically significant differences were found between MS medium and Rugini OM medium in terms of shoot length and vigour with this method of culture. When using the conventional method, better results can be achieved with MS medium. This research can be considered as a first step towards the production of Chlorophytum comosum (Thunb.) Jacques on a larger scale.