Author:
Elmanfe Galal,Omar Suad K.,Abdolla Noreldin S.Y.,Hassan Amna M.
Abstract
The aim of the present study was to evaluate the levels of nicotine in twenty urine samples taken from ten smokers and ten non-smokers in Libya. Each volunteer was required to complete a questionnaire before providing the urine sample. The evaluation of the nicotine concentrations was carried out by means of a simple, rapid, cost effective but reliable, one-step extraction technique-reversed-phase high-performance liquid chromatography which was developed and validated for this purpose. The criteria and factors taken into consideration for this evaluation and validation include the linearity, precision, accuracy, limit of detection, and limit of quantitation. The urine samples from the smokers presented nicotine concentrations in the range of 0.037-1.979 µg/ml, with an average of 0.663 µg/ml. The range of the nicotine concentrations in non-smokers, on the other hand, was from 0.017-1.331 g/ml, where 0.273 µg/ml is the average value. Statistical analyses show that the nicotine concentrations were very significant in the smoker samples in contrast with the nonsmoker samples
Publisher
Omar Al-Mukhtar University
Reference23 articles.
1. Allen, J. A. V., D. Haviland, M. L. Healton, C. Davis, K. C. Farrelly, M. C. Husten, & C. G. Pechacek, T. (2003). Tobacco use among middle and high school students, United States, 2002. Morbidity and Mortality Weekly Report, 52(1096), 1096.
2. Ceppa, F., El Jahiri, Y., Mayaudon, H., Dupuy, O., & Burnat, P. (2000). High performance liquid chromatographic determination of cotinine in urine in isocratic mode. Journal of Chromatography B: Biomedical Sciences and Applications, 746(2), 115-122.
3. Davoli, E., Stramare, L., Fanelli, R., Diomede, L., & Salmona, M. (1998). Rapid solid-phase extraction method for automated gas chromatographic–mass spectrometric determination of nicotine in plasma. Journal of Chromatography B: Biomedical Sciences and Applications, 707(1), 312-316.
4. Elmanfe, G., & Abdulla, A. (2014). Spectrophotometric Determination of Nicotine in Some Libyan Smokers’ Blood and Compared with Nonsmokers. Paper presented at the 1st Conference on Chemical Sciences and its Applications, 21-23 April 2014, Elbaida-Libya
5. Giovino, G. A. (1999). Epidemiology of tobacco use among US adolescents. Nicotine and Tobacco Research 1, (Suppl 1):, S31-40.
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