The Effects of Sorghum bicolor Aqueous Leaf Sheaths Extract on Some Selected Biochemical Parameters of Phenylhydrazine-Induced Hemoltyic Anemic Male Wistar Rats

Abstract

In this study the effect of Sorghum bicolor was tested on some selected biochemical parameters (direct, total and indirect billirubin, creatinine, total protein, serum albumin and urea) was appraised to determine if the plant extract can reverse the aberrance in the values of these parameters. Study design: 40 experimental animals were divvied into four groups and hemolytic anemia was induced in three of the groups with two of the groups given the herbal extract in varying degree (low and high dosage) to assess the efficacy of the drug on alleviating selected biochemical parameters, to ultimately investigate the curative effect of the botanical extract on hemolytic anemia. Place and Duration of Study: Cell and Tissue Culture Research Laboratory (Drug Discovery Unit), Department of Biochemistry, Lagos State University between September 2020 and October 2020. Methodology: 40 male Wistar rats were divvied into four equal groups: normal, anemic untreated, anemic low dosed and anemic high dosed. The low dosed and high dosed rats were given the extract at 100mg/kg and 500mg/kg respectively. After 7 days of extract administration, the rats were sacrificed and blood samples taken though heart puncture were centrifuged for sera which was assayed using Randox Laboratories kit. Result: The highest levels of bilirubin, creatinine and urea level and lowest levels of total protein and albumin were recorded in the untreated rats, indicating hemolysis. The treated rats recorded a significant dosedependent reduction in the bilirubin levels and a significant increase in the total protein levels of the two treated rats groups. Conclusion: this study revealed S. bicolor extract to be a potential amelioration of hemolytic anemia.