Metronidazole Analysis: Method Development and Validation by using UV Spectroscopy

Abstract

The current study examines the creation of UV Spectrophotometric technique for metronidazole. Metronidazole in tablet dosage form can now be estimated using an easy to use, precise and economical spectrophotometric approach. The ideal circumstances were determined for medication analysis. It was discovered that 275 nm was the maximum wavelength (λmax). Metronidazole percentage recovery fell between 98-102%. In the 5-40 μg/ml concentration range, beers law followed. A true correlation between optical density and intensity can be seen in calibration curve. Linearity, accuracy, precision, LOD, and LOQ were all validated in accordance with ICH requirements.