Development and Validation of a UV- Visible Method for Determining Piperine in Bulk and Formulation

Abstract

Standardization of herbal preparations is crucial because the growing use of herbal medicines and the globalization of the herbal medicinal industries have raised serious concerns about safety among the public and health authorities. However, the majority of the time, it was discovered that the standardization procedure was challenging and time-consuming. Thus, piperine from Piper nigrum in the commercial formulation could be quantitatively determined and standardized using an easy-to-use and trustworthy UV-Visible Spectrophotometric technique. In accordance with WHO guidelines, UV-visible spectrophotometric investigations of several parameters are part of the study. This work presents a spectrophotometric technique in the UV area at 342 nm that is easy to use, quick, sensitive and accurate. It was established how much piperine was present in the sample and validated. Therefore, piperine in the commercial formulations may be reliably quantified using the suggested method.