Using a Rapid, Accurate, and Cost-Effective QiaXcel Advanced Platform for Microsatellite Instability Detection in Colorectal Carcinoma in North of Iraq

Abstract

Objective: The objective of this investigation was to address the limitations of the most popular microsatellite instability (MSI) detectionmethod, which uses fluorescent capillary sequencers. Methods: Using the QiaXcel Advanced system (Qiagen, Hilden, Germany) based on capillary electrophoresis, the MSI status of 53 IraqiFormalin Fixed Paraffin Embedded (FFPE) CRC samples was examined. BAT25, BAT26, BAT40, D2S123, D5S346, D17S250, NR21, NR22, NR27,Mycl1, TPOX, and TH01 were among the panel of twelve polymorphic markers that were used. Results: Using a QiaXcel Advanced platform was successfully established to determine the MSI status. Among 53 cases of CRC, MSI wasobserved in 12 cases (22.64%) who had MSI-H. Conclusion: Due to MSI’s significance in the progression of cancer, this quick and inexpensive PCR-based technique can enhance theclinical management of CRC, which may further alter the patient’s outcome.