Functional Profiling of Transcription Factor Genes in Neurospora crassa

Author:

Carrillo Alexander J12,Schacht Patrick13,Cabrera Ilva E13,Blahut Johnathon4,Prudhomme Loren4,Dietrich Sarah1,Bekman Thomas1,Mei Jennifer4,Carrera Cristian4,Chen Vivian4,Clark Isaiah4,Fierro Gerardo4,Ganzen Logan4,Orellana Jose4,Wise Shelby4,Yang Kevin4,Zhong Hui4,Borkovich Katherine A1234

Affiliation:

1. Department of Plant Pathology and Microbiology

2. Graduate Program in Microbiology

3. Graduate Program in Genetics, Genomics and Bioinformatics, and

4. College of Natural and Agricultural Sciences, University of California, Riverside, California 92521

Abstract

Abstract Regulation of gene expression by DNA-binding transcription factors is essential for proper control of growth and development in all organisms. In this study, we annotate and characterize growth and developmental phenotypes for transcription factor genes in the model filamentous fungus Neurospora crassa. We identified 312 transcription factor genes, corresponding to 3.2% of the protein coding genes in the genome. The largest class was the fungal-specific Zn2Cys6 (C6) binuclear cluster, with 135 members, followed by the highly conserved C2H2 zinc finger group, with 61 genes. Viable knockout mutants were produced for 273 genes, and complete growth and developmental phenotypic data are available for 242 strains, with 64% possessing at least one defect. The most prominent defect observed was in growth of basal hyphae (43% of mutants analyzed), followed by asexual sporulation (38%), and the various stages of sexual development (19%). Two growth or developmental defects were observed for 21% of the mutants, while 8% were defective in all three major phenotypes tested. Analysis of available mRNA expression data for a time course of sexual development revealed mutants with sexual phenotypes that correlate with transcription factor transcript abundance in wild type. Inspection of this data also implicated cryptic roles in sexual development for several cotranscribed transcription factor genes that do not produce a phenotype when mutated.

Publisher

Oxford University Press (OUP)

Subject

Genetics(clinical),Genetics,Molecular Biology

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