Identification of New Players in Cell Division, DNA Damage Response, and Morphogenesis Through Construction of Schizosaccharomyces pombe Deletion Strains-Reference-Cited by-同舟云学术

Identification of New Players in Cell Division, DNA Damage Response, and Morphogenesis Through Construction of Schizosaccharomyces pombe Deletion Strains

Published:2015-03-01 Issue:3 Volume:5 Page:361-370
ISSN:2160-1836
Container-title:G3 Genes|Genomes|Genetics
language:en
Short-container-title:

Author:

Chen Jun-Song¹,Beckley Janel R¹,McDonald Nathan A¹,Ren Liping¹,Mangione MariaSanta¹,Jang Sylvia J¹,Elmore Zachary C¹,Rachfall Nicole¹,Feoktistova Anna¹,Jones Christine M¹,Willet Alaina H¹,Guillen Rodrigo¹,Bitton Danny A²,Bähler Jürg²,Jensen Michael A³,Rhind Nick⁴,Gould Kathleen L¹¹

Affiliation:

1. Department of Cell and Developmental Biology, Vanderbilt University School of Medicine, Nashville, Tennessee 37232

2. Department of Genetics, Evolution and Environment and UCL Cancer Institute, University College London, London WC1E 6BT, United Kingdom

3. Genome Technology Center, Stanford University School of Medicine, Palo Alto, California 01605

4. Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Worcester, Massachusetts 01605

Abstract

Abstract Many fundamental biological processes are studied using the fission yeast, Schizosaccharomyces pombe. Here we report the construction of a set of 281 haploid gene deletion strains covering many previously uncharacterized genes. This collection of strains was tested for growth under a variety of different stress conditions. We identified new genes involved in DNA metabolism, completion of the cell cycle, and morphogenesis. This subset of nonessential gene deletions will add to the toolkits available for the study of biological processes in S. pombe.

Publisher

Oxford University Press (OUP)

Subject

Genetics (clinical),Genetics,Molecular Biology

Link

http://academic.oup.com/g3journal/article-pdf/5/3/361/37191997/g3journal0361.pdf

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