Removal of the Bloom Syndrome DNA Helicase Extends the Utility of Imprecise Transposon Excision for Making Null Mutations in Drosophila-Reference-Cited by-同舟云学术

Removal of the Bloom Syndrome DNA Helicase Extends the Utility of Imprecise Transposon Excision for Making Null Mutations in Drosophila

Published:2009-11-01 Issue:3 Volume:183 Page:1187-1193
ISSN:1943-2631
Container-title:Genetics
language:en
Short-container-title:

Author:

Witsell Alice¹,Kane Daniel P¹,Rubin Sarah¹,McVey Mitch¹²

Affiliation:

1. Department of Biology, Tufts University, Medford, Massachusetts 02155 and

2. Program in Genetics, Tufts Sackler School of Graduate Biomedical Sciences, Boston, Massachusetts 02111

Abstract

Abstract Transposable elements are frequently used in Drosophila melanogaster for imprecise excision screens to delete genes of interest. However, these screens are highly variable in the number and size of deletions that are recovered. Here, we show that conducting excision screens in mus309 mutant flies that lack DmBlm, the Drosophila ortholog of the Bloom syndrome protein, increases the percentage and overall size of flanking deletions recovered after excision of either P or Minos elements.

Publisher

Oxford University Press (OUP)

Subject

Genetics

Link

https://academic.oup.com/genetics/article-pdf/183/3/1187/42173847/genetics1187.pdf

Reference42 articles.

1. Mobilization of a Minos Transposon in Drosophila melanogaster Chromosomes and Chromatid Repair by Heteroduplex Formation

2. The Drosophila melanogaster sir2+ Gene Is Nonessential and Has Only Minor Effects on Position-Effect Variegation

3. Mobile D-loops are a preferred substrate for the Bloom's syndrome helicase

4. Drosophila IRBP/Ku p70 corresponds to the mutagen-sensitive mus309 gene and is involved in P-element excision in vivo.

5. Drosophila P-element transposase is a novel site-specific endonuclease

Cited by 17 articles. 订阅此论文施引文献订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献

1. Methods for Creating Fly Models to Understand the Molecular Mechanisms Underlying Neurological Diseases;Insights into Human Neurodegeneration: Lessons Learnt from Drosophila;2019

2. Dissection of Nidogen function in Drosophila reveals tissue-specific mechanisms of basement membrane assembly;PLOS Genetics;2018-09-27

3. In vivo bioassay to test the pathogenicity of missense human AIP variants;Journal of Medical Genetics;2018-04-09

4. dOCRL maintains immune cell quiescence by regulating endosomal traffic;PLOS Genetics;2017-10-13

5. P Transposable Elements in Drosophila and other Eukaryotic Organisms;Mobile DNA III;2015-05-26