Affiliation:
1. Department of Biological Sciences, Graduate School of Science, Osaka University, Toyonaka 560-0043, Japan
2. Department of Biological Science and Technology, Graduate School of Industrial Science and Technology, Tokyo University of Science, 125-8585, Japan
Abstract
Abstract
Notch signaling plays crucial roles in intercellular communications. In Drosophila, the pecanex (pcx) gene, which encodes an evolutionarily conserved multi-pass transmembrane protein, appears to be required to activate Notch signaling in some contexts, especially during neuroblast segregation in the neuroectoderm. Although Pcx has been suggested to contribute to endoplasmic reticulum homeostasis, its functions remain unknown. Here, to elucidate these roles, we performed genetic modifier screens of pcx. We found that pcx heterozygotes lacking its maternal contribution exhibit cold-sensitive lethality, which is attributed to a reduction in Notch signaling at decreased temperatures. Using sets of deletions that uncover most of the second and third chromosomes, we identified four enhancers and two suppressors of the pcx cold-sensitive lethality. Among these, five genes encode known Notch-signaling components: big brain, Delta (Dl), neuralized (neur), Brother of Bearded A (BobA), a member of the Bearded (Brd) family, and N-ethylmaleimide-sensitive factor 2 (Nsf2). We showed that BobA suppresses Dl endocytosis during neuroblast segregation in the neuroectoderm, as Brd family genes reportedly do in the mesoderm for mesectoderm specification. Analyses of Nsf2, a key regulator of vesicular fusion, suggested a novel role in neuroblast segregation, which is distinct from Nsf2’s previously reported role in imaginal tissues. Finally, jim lovell, which encodes a potential transcription factor, may play a role in Notch signaling during neuroblast segregation. These results reveal new research avenues for Pcx functions and Notch signaling.
Publisher
Oxford University Press (OUP)
Reference99 articles.
1. The BTB/POZ domain: a new protein-protein interaction motif common to DNA- and actin-binding proteins.;Albagli;Cell Growth Differ.,1995
2. Gravitaxis in Drosophila melanogaster: a forward genetic screen.;Armstrong;Genes Brain Behav.,2006
3. Notch signaling.;Artavanis-Tsakonas;Science,1995
4. Notch signaling: cell fate control and signal integration in development.;Artavanis-Tsakonas;Science,1999
Cited by
3 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献