The Hermes Transposon of Musca domestica Is an Efficient Tool for the Mutagenesis of Schizosaccharomyces pombe-Reference-Cited by-同舟云学术

The Hermes Transposon of Musca domestica Is an Efficient Tool for the Mutagenesis of Schizosaccharomyces pombe

Published:2007-12-01 Issue:4 Volume:177 Page:2519-2523
ISSN:1943-2631
Container-title:Genetics
language:en
Short-container-title:

Author:

Evertts Adam G¹,Plymire Christopher¹,Craig Nancy L²,Levin Henry L¹

Affiliation:

1. Section on Eukaryotic Transposable Elements, Laboratory of Gene Regulation and Development, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892 and

2. Department of Molecular Biology and Genetics, Howard Hughes Medical Institute and Johns Hopkins School of Medicine, Baltimore, Maryland 21205

Abstract

Abstract Currently, no transposon-based method for the mutagenesis of Schizosaccharomyces pombe exists. We have developed such a system based on the introduction of the hermes transposon from the housefly into S. pombe. This system efficiently disrupts open reading frames and allows the insertion sites to be readily identified.

Publisher

Oxford University Press (OUP)

Subject

Genetics

Link

https://academic.oup.com/genetics/article-pdf/177/4/2519/42187342/genetics2519.pdf

Reference18 articles.

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2. TATA box mutations in the Schizosaccharomyces pombe nmt1 promoter affect transcription efficiency but not the transcription start point or thiamine repressibility

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4. The BDGP Gene Disruption Project

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