Highly Efficient Genome Modifications Mediated by CRISPR/Cas9 in Drosophila-Reference-Cited by-同舟云学术

Highly Efficient Genome Modifications Mediated by CRISPR/Cas9 in Drosophila

Published:2013-09-01 Issue:1 Volume:195 Page:289-291
ISSN:1943-2631
Container-title:Genetics
language:en
Short-container-title:

Author:

Yu Zhongsheng¹²,Ren Mengda³,Wang Zhanxiang⁴,Zhang Bo⁴,Rong Yikang S⁵,Jiao Renjie¹,Gao Guanjun³

Affiliation:

1. State Key Laboratory of Brain and Cognitive Science, Institute of Biophysics, Chinese Academy of Sciences, Datun Road 15, Beijing 100101, China

2. University of Chinese Academy of Sciences, Beijing 100080, China

3. School of Life Sciences, Tsinghua University, Beijing 100084, China

4. Key Laboratory of Cell Proliferation and Differentiation of the Ministry of Education, College of Life Sciences, Peking University, Beijing 100871, China

5. Laboratory of Biochemistry and Molecular Biology, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892

Abstract

Abstract We report that Cas9/gRNA mediates efficient genetic modifications in Drosophila. Through targeting seven loci, we achieved a germline efficiency of up to 100%. Genes in both heterochromatin and euchromatin can be modified efficiently. Thus the Cas9/gRNA system is an attractive tool for rapid disruption of essentially any gene in Drosophila.

Publisher

Oxford University Press (OUP)

Subject

Genetics

Link

https://academic.oup.com/genetics/article-pdf/195/1/289/42117158/genetics0289.pdf

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