Affiliation:
1. Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115
Abstract
Abstract
Here we describe a simple method for generating donor vectors suitable for targeted transgenesis via recombinase-mediated cassette exchange (RMCE) using the ΦC31 integrase. This PCR-based strategy employs small attB “tails” on the primers used to amplify a sequence of interest, permitting the rapid creation of transgenes for in vivo analysis.
Publisher
Oxford University Press (OUP)
Cited by
26 articles.
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