Unexpected Expression Pattern of Tetracycline-Regulated Transgenes in Mice-Reference-Cited by-同舟云学术

Unexpected Expression Pattern of Tetracycline-Regulated Transgenes in Mice

Published:2009-04-01 Issue:4 Volume:181 Page:1687-1691
ISSN:1943-2631
Container-title:Genetics
language:en
Short-container-title:

Author:

Bao-Cutrona M¹²,Moral P¹

Affiliation:

1. Department of Animal Biology-Anthropology, Faculty of Biology, University of Barcelona, 08028 Barcelona, Spain and

2. Department of Neuroscience, Istituto di Ricerche Farmacologiche ‘Mario Negri’, 20156 Milan, Italy

Abstract

Abstract In generating a conditional transgenic murine model based on a tetracycline-regulated system, we obtained unexpected patterns of expression due to the transcriptional inactivity of the tet-responder promoter. Here we show strong cell-type-restricted expression that was variegated to an extent determined by the number of responder transgene copies integrated into the host genome.

Publisher

Oxford University Press (OUP)

Subject

Genetics

Link

https://academic.oup.com/genetics/article-pdf/181/4/1687/42091076/genetics1687.pdf

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