cnd-1/NeuroD1 Functions with the Homeobox Gene ceh-5/Vax2 and Hox Gene ceh-13/labial To Specify Aspects of RME and DD Neuron Fate in Caenorhabditis elegans

Author:

Aquino-Nunez Wendy,Mielko Zachery E,Dunn Trae,Santorella Elise M,Hosea Ciara,Leitner Lauren,McCalla Derrica,Simms Claire,Verola Wendy M,Vijaykumar Sharanya,Hudson Martin L

Abstract

Abstract Identifying the mechanisms behind neuronal fate specification are key to understanding normal neural development in addition to neurodevelopmental disorders such as autism and schizophrenia. In vivo cell fate specification is difficult to study in vertebrates. However, the nematode Caenorhabditis elegans, with its invariant cell lineage and simple nervous system of 302 neurons, is an ideal organism to explore the earliest stages of neural development. We used a comparative transcriptome approach to examine the role of cnd-1/NeuroD1 in C. elegans nervous system development and function. This basic helix-loop-helix transcription factor is deeply conserved across phyla and plays a crucial role in cell fate specification in both the vertebrate nervous system and pancreas. We find that cnd-1 controls expression of ceh-5, a Vax2-like homeobox class transcription factor, in the RME head motorneurons and PVQ tail interneurons. We also show that cnd-1 functions redundantly with the Hox gene ceh-13/labial in defining the fate of DD1 and DD2 embryonic ventral nerve cord motorneurons. These data highlight the utility of comparative transcriptomes for identifying transcription factor targets and understanding gene regulatory networks.

Publisher

Oxford University Press (OUP)

Subject

Genetics (clinical),Genetics,Molecular Biology

Reference57 articles.

1. Vax2 regulates retinoic acid distribution and cone opsin expression in the vertebrate eye.;Alfano;Development,2011

2. Detecting differential usage of exons from RNA-seq data.;Anders;Genome Res.,2017

3. Andrews, S , 2010 FastQC: a quality control tool for high throughput sequence data. https://www.bioinformatics.babraham.ac.uk/projects/fastqc/

4. The AMP-activated protein kinase AAK-2 links energy levels and insulin-like signals to lifespan in C. elegans.;Apfeld;Genes Dev.,2004

5. All in the family: proneural bHLH genes and neuronal diversity.;Baker;Development,2018

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