Effect of Contamination by Cleaning Agents and Hemostatic Agent on Dentin Bond Strength

Abstract

BACKGROUND Contamination by hemostatic agent and rinsing with different disinfectant solutions can influence on micro shear bond strength between dentin and resin composite. The major objective of this study was to investigate the micro shear bond strengths of an etch and rinse technique and self-etch technique to human dentin contaminated with a hemostatic agent containing AlCl3 followed by chlorhexidine (CHX) and boric acid cleaning. METHODS A 50 non-carious, permanent, third molar teeth were randomly divided into five groups (N = 10 per group); Group 1: Self etch technique (OptiBond All - In - One, Kerr) was used on dentin contaminated with hemostatic agent (Alustat, Cerkamed). Group 2: Etch and rinse technique (Phosphoric acid then OptiBond Universal, Kerr) was used. Group 3: Contaminated dentin was cleaned with CHX for 60 seconds and then self-etch technique was used. Group 4: Contaminated dentin was cleaned with boric acid for 60 seconds and then self-etch technique was used. Group 5: Hemostatic agent was not applied, only self-etch technique was used. Flat occlusal dentin surfaces were prepared and then restored with resin composite rod (Herculite Classic, Kerr) (1 mm diameter × 1 mm height). After thermocycling (1,000 cycles), composite sticks were subjected to microshear until fracture occurred at a crosshead speed of 0.5 mm / min. Statistical analysis was made with one-way analysis of variance (ANOVA) and multiple comparison tests with Tukey. RESULTS The mean microshear bond strength values of group 1 to group 5 were 21.89 ± 12.43, 26.15 ± 22.79, 20.05 ± 12.03, 35.08 ± 13.20 and 19.41 ± 6.53 MPa, respectively. When the values of the groups were compared, the difference was not significant. (P = 0.108). CONCLUSIONS Boric acid and CHX may be used as alternatives to other materials that are used as cavity disinfectants on contaminated dentin by hemostatic agent. KEY WORDS Boric Acid, CHX, Hemostatic Agent, Adhesive Systems, Shear Bond Strength