Abstract
Treatment of wounds resulting from combat injuries is complicated by the addition of a secondary infection caused by multi-resistant bacteria. The liquid patch appears to be a promising formulation for wound therapy. The antimicrobial effect of essential oils is manifested due to the content of numerous components that interfere with the proliferation and formation of bacterial resistance.
The aim of the work was to substantiate the choice of Origanum vulgare L. essential oil as the main active ingredient of a liquid patch with antimicrobial action, confirm the composition of the essential oil and study the antimicrobial properties of the model drug.
The object of the study were model samples of a liquid patch with an experimentally developed film-forming system. Various concentrations of commercial essential oil of O. vulgare L., the composition of which was confirmed by GB/MS analysis, were used as the active pharmaceutical ingredient. Antimicrobial activity was studied by direct contact method using microbial culture tests.
The use of liquid patch in the treatment of skin lesions of various etiologies, including combat wounds, is justified. The variability of the composition of the essential oil of O. vulgare L. depending on the subspecies, chemotype and growth conditions of the plant is discussed. The component composition of a commercial sample of O. vulgare L. essential oil, confirmed by GC/MS analysis, is considered. 11 components declared by the manufacturer were found to be completely identical, in particular, carvacrol, thymol, and monoterpene hydrocarbons. The other 11 compounds discovered were monoterpenoids and sesquiterpenoids. Research has confirmed the feasibility of using O. vulgare L. essential oil, thymol-carvacrol chemotype, as an antimicrobial component. The dependence of the antimicrobial activity of model samples of liquid plaster on the concentration of Oregano essential oil was established.
The essential oil of O. vulgare L. has a variable composition and requires mandatory control of the content of thymol and carvacrol, which inhibit the growth of the studied microorganisms. The antimicrobial effect of a liquid patch containing essential oil of O. vulgare L. at a concentration of 0.15 mg/ml is manifested against test cultures of microorganisms – Escherichia coli, Klebsiella pneumoniae, Staphylococcus aureus, Enterococcus faecalis.
Publisher
The State Expert Center of the Ministry of Health of Ukraine
Reference33 articles.
1. Lu M., Dai T., Murray C. K., Wu M. X. Bactericidal Property of Oregano Oil Against Multidrug-Resistant Clinical Isolates // Frontiers in Microbiology. – 2021. – V. 12 – P. 713573. https://doi.org/10.3389/fmicb.2021.713573
2. Trampuz A., Pidgaiska O., Filipenko V. et al. Pidkhody do khirurhichnoho likuvannia ta antybakterialnoi terapii khvorykh na khronichnu infektsiіu pislia boiovykh poranen // Ortopedia, travmatolohia ta protezuvannia. – 2023. – № 3. – S. 62–68.
3. Zheliba M. D., Verba A. V., Bogush H. L. ta in. Mikrobiolohichni aspekty ranovoi infektsii u poterpilykh vnaslidok boiovykh dii ta yii kompleksne likuvannia z zastosuvanniam vakuum terapii // Suchasni medychni tekhnolohii. – 2019. – № 3. – S. 50–54.
4. Shaprynskii V. O., Verba A. V., Shaprynskii Ye. V. ta in. Osoblyvosti likuvannia hniino-zapalnukh uskladnen pry vohnepalnykh poranenniakh kintsivok // Kharkivska khirurhichna shkola. – 2021. – № 2. – S. 121–127.
5. Derzhavna farmakopeia Ukrainy / DP «Ukrainskii naukovyi farmakopeinyi tsentr yakosti likarskikh zasobiv». 2-e vyd. Dop. 4. – Kharkiv: DP «Ukrainskyi naukovyi farmakopeinyi tsentr yakosti likarskikh zasobiv», 2020. – 600 s.