Green Synthesis of Silver Nanoparticles Using Safran (Crocus sativus) Purple flower and Their Antimicrobial activity

Abstract

Objective: In this research, CS-AgNPs were created, described, and their antibacterial activity assessed utilizing an inexpensive, simple, and ecologically friendly extraction approach from the waste saffron flower's purple flower parts. Methods: : In this study, silver nanoparticles (AgNPs) were synthesized quickly, cheaply, and environmentally friendly utilizing purple flower extract from saffron (Crocus sativus (CS)). For the explanation of the structure of silver nanoparticles synthesized with saffron flower extract (CS-AgNPs); UV-visible (UV-vis.) Spectrophotometer, Fourier Scanning Electron Microscope (FE-SEM), Scanning Electron Microscope (SEM), Electron Distributed X-rays (EDX), Fourier Transform Infrared Spectroscopy (FT-IR), X-Ray Characterized using Diffraction Diffractrometer (XRD), Transmission Electron Microscope (TEM), Zeta potential data. It was discovered that the UV-visible spectrum of the biologically produced silver nanoparticles generated at a maximal wavelength of 405.68 nm in the spectrum collected after 15 minutes. Furthermore, it was noted that the synthesized nanoparticles' UV spectrum, taken a month later, revealed the same wavelength and that the resulting nanoparticles were stable. CS-AgNPs' antimicrobial effects against gram-positive, gram-negative, and fungal pathogens were assessed employing the minimum inhibition concentration approach. Results: The generated CS-AgNPs were found to be active against both fungi and bacteria as a consequence. Conclusion: It turned out that even at very low concentrations, the antimicrobial activity of silver nanoparticles enhanced with decreasing size and had high antibacterial and anticandidal implementation.