Cytotoxic activity and DNA fragmentation study of nano structured assemblies of copper sulfide nanoparticles using single route molecular precursor source of copper

Abstract

In this paper, we have reported an easy, template free and squashy solution chemistry path was applied to synthesize variegated nanostructured congregation assemblies of copper nanoparticles at room temperature using hydrazinecarbothioamide coordinated Cu(II) complex through a single route molecular precursor source. The parent compounds were heated under microwave irradiation to obtain diversified CuS nanostructures in the shapes of spheres and nanotubes were found to be assemblies of either nanoplates or nanoparticles. The formation of nanostructured CuS nano particles was detailed studied by differing the synthetic conditions such as reaction time, temperature, parent compounds ratio, and the presence of counter ions. The presence of NO3 - and SO4 2- ions as counter ions were found to be suitable for the formation of nanotubes whereas the presence of Clions initiate the formation of spherical nano assemblies of CuS nano material is obtained. The synthesized samples were characterized using various structural, morphological and optical characterization techniques like elemental analysis, FT-IR, UV-Vis, and thermogravimetric analysis/differential thermogravimetric (TGA/DG). Important changes were observed in the FT-IR spectra of the copper complex compared to the FT-IR spectrum of ligand. X-ray diffraction studies confirms the formation of hexagonal crystalline phase of CuS nano particles: Transmission electron microscopy exhibit nanotube like structures with an average particle size of 78nm. Strong UV absorption band at 425nm confirms the formation of good quality CuS nano particles. The microwave irradiation of parent compounds in the presence of nonpolar solvents like DMF, DMSO environment played a significant role in decreasing the reaction time, decreases the possibility of side reactions and proceeds the reaction in the formation of good quality nanoparticles. The SEM analysis of CuS nano particles confirms the agglomerated grain like surface morphology of nanoparticles. All compounds showed the various pharmacological activities like antioxidant, antibacterial activities due to the presence of strong electron withdrawing and electron releasing functional groups are present in molecular precursors. The microwave synthesis of CuS nanostructured assemblies in nonpolar solvent in a proper stoichiometric ratio is an excellent method for preparing highly efficient bio active agents like antibacterial & antioxidant agents which can be considered as a good drug candidate for medicinal chemists in future.