Impact of DC glow discharge plasma treated Ti doped hydroxyapatite nanomaterials using antibacterial and cytotoxicity applications

Abstract

In this work we developed Ti doped hydroxyapatite with were synthesized by using a solgel technique with exposed to DC glow discharge plasma with different times treated. The pure and Ti/HAp were characterised by using different characterizations like XRD, FTIR, SEM, EDAX, UV-visible, PL, TG-DTA, antibacterial activity, 3T3 fibroblast cells and MCF-7 breast cancer cell line with cytotoxity efficiency analysis. The pure HAp and Ti/HAp materials had a nanocube and nanoflakee shape in the SEM results. In the ultraviolet-visible light (UV-vis) spectrum, the band edge absorption of bare HAp and Ti/HAp samples is discovered at wavelengths of 295 nm and 328 nm, respectively. The antibacterial activity of untreated and plasma-treated pure HAP and Ti/HAp samples are tested against two gram-positive bacteria, Bacillus cereus and Staphylococcus aureus, as well as two gram-negative bacteria, E. coli and Pseudomonas aeruginosa, using the agar well diffusion technique. The MTT assay was used to investigate the mean per cent (% percent) cell survival of Ti/HAp at various concentrations (6 mg/mL; 85 mg/mL) against 3T3 fibroblast cells and human MCF-7 Breast cancer cell lines. The synthesised Ti/HAp biocompatibility makes it a viable contender for future biological uses.