Features of proteomic profi le of blood serum of children with bronchial asthma who underwent perinatal central nervous system damage-Reference-Cited by-同舟云学术

Features of proteomic profi le of blood serum of children with bronchial asthma who underwent perinatal central nervous system damage

Published:2019-12-26 Issue:4 Volume:10 Page:59-65
ISSN:2618-7876
Container-title:Medical Herald of the South of Russia
language:
Short-container-title:Med. vestn. Ûga Ross.

Author:

Lebedenko A. A.¹^ORCID,Afonin A. A.¹^ORCID,Loginova I. G.¹,Semernik O. E.¹^ORCID,Gunko V. O.¹^ORCID,Alliluev I. A.¹^ORCID,Larichkin A. V.¹^ORCID

Affiliation:

1. Rostov State Medical University

Abstract

Objective: proteomic analysis of blood serum of children with bronchial asthma with a history of indication of the pathology of the perinatal period.Materials and methods: a comprehensive examination of children suffering from asthma and patients of the control group using proteomic analysis of blood serum was carried out. Proteomic analysis of blood serum was performed using two-dimensional high-resolution gel electrophoresis followed by staining of gels with fl uorescent dye and time-of-fl ight MALDI mass spectrometry. The proteins were identifi ed using Mascot MS peptide fi ngerprint analysis algorithm and Swiss-Prot protein database.Results: comparative analysis of the proteomic profi le of blood serum of children with pathology of the central nervous system in the perinatal period and developed in the subsequent bronchial asthma and control group patients allowed to establish that number proteins products were reduced in this disease. The proteins in the molecular mass range 16-33 kDa (p<0.05) were identifi ed: glutathione peroxidase 3, transthyretin and system components compliment С4В and C3.Conclusion: modifi cation of the production of these proteins indicates the presence of damage at the molecular level, leading to metabolic and functional disorders in the body of a patient with asthma having a history of indicating the pathology of the perinatal period.

Publisher

Rostov State Medical University

Subject

General Earth and Planetary Sciences,General Environmental Science

Reference21 articles.

1. Trepilec SV, Golosnaya GS, Trepilec VM, Kukushkin EI. Hypoxic hemorrhagic brain lesions in neonates: the significance of determination of neurochemical markers, inflammation markers and apoptosis in the neonatal period and catamnesis follow-up results. Pediatriya. 2018;97(1):31-37. (In Russ).

2. Krasnoruckaya ON, Ledneva VS. Clinical and biochemical indices in diagnosis of developmental disorders in children with consequences of perinatal lesion of the nervous system. Pediatriya named after G. N. Speransky. 2018;97(3):175-179. (In Russ).

3. SHnitkova EV, Filosofova MS, CHemodanov VV, Sokolova SV, Pronina II, Baldaev AA. Osobennosti ontogeneticheskogo razvitiya detej, perenesshih perinatal’nuyu gipoksiyu. Vestnik Ivanovskoj medicinskoj akademii. 2007;12(3-4):194- 195. (In Russ).

4. Pavlenko VA, Mel’nikova IM, Mizernickij YUL. The prognostic significance of functional parameters of the respiratory system and autonomic nervous system in infants with a history of acute obstructive bronchitis against the background of perinatal lesion of the central nervous system. Voprosy prakticheskoj pediatrii. 2015;10(1):7-14. (In Russ).

5. National program “Bronchial asthma in children. Th e strategy of treatment and prevention “5th ed., Corr. and add. M .: Izd. House “Atmosphere”; 2017. (In Russ).