Evaluation of markers of apoptotic processes and peroxide oxidation of lipids of the mitochondrial fraction of the liver of animals carrying Lewis epidermoid carcinoma at different stages of the development of the tumor process with the introduction of organotin compounds with a tread fragment

Abstract

Objective: to evaluate changes in markers of apoptotic processes and lipid peroxidation (POL) by accumulation of malondialdehyde (MDA) in the mitochondrial fraction of the liver of animals carrying Lewis epidermoid carcinoma at different stages of the tumor process with the introduction of bis-(3,5-di-tert-butyl-4-hydroxyphenyl)dimethylolol thiolate (Me-3) and (3,5-di-tert-butyl-4-hydroxyphenyl)triphenylololate (Me-5). Materials and methods: the work was performed using laboratory animals - female mice of the C57Bl line/6. 48 hours after the Lewis epidermoid carcinoma strain was transplanted, substances Me-3 and Me-5 were administered once a day for 5 days intraperitoneally at the maximum effective dose of 375 mg/kg and 250 mg/kg, respectively. Animals of the control group were injected with a carrier in similar modes and volumes. Results: when Me-3 was administered at the maximum effective dose on days 7 and 21, a decrease in the level of all the studied indicators was noted, which indicates a high actioxidant activity of a hybrid organotin compound containing one tin-containing [-Sn(CH3)2] and two protective antioxidant fragments (3,5-di-tert-butyl-4-hydroxyphenyl). The compound Me-5 has a more pronounced prooxidant potential, as evidenced by high levels of damage to mitochondrial DNA (8–hydroxy–2'–deoxyguanosine) and malonic dialdehyde. Conclusion: the introduction of bis-(3,5-di-tert-butyl-4-hydroxyphenyl)dimethylolol (Me-3) and (3,5-di-tert-butyl-4-hydroxyphenyl)triphenylolol (Me-5) compounds revealed a change in the pro/antioxidant state and the launch of apoptotic processes in liver cells.