High-performance serial block-face SEM of nonconductive biological samples enabled by focal gas injection-based charge compensation

Author:

DEERINCK T.J.1,SHONE T.M.1,BUSHONG E.A.1ORCID,RAMACHANDRA R.1,PELTIER S.T.1,ELLISMAN M.H.1234

Affiliation:

1. Center for Research in Biological Systems and the National Center for Microscopy and Imaging Research; University of California, San Diego; La Jolla California U.S.A.

2. Department of Neurosciences; University of California at San Diego, La Jolla; San Diego California U.S.A.

3. Salk Institute for Biological Studies; La Jolla California U.S.A.

4. Janelia Research Campus; Howard Hughes Medical Institute; Ashburn Virginia U.S.A.

Funder

National Center for Microscopy and Imaging Research

National Institute of General Medical Sciences

Publisher

Wiley

Subject

Histology,Pathology and Forensic Medicine

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