The neuropeptide pigment‐dispersing factor signals independently of Bruchpilot‐labelled active zones in daily remodelled terminals of Drosophila clock neurons

Abstract

AbstractThe small ventrolateral neurons (sLNvs) are key components of the central clock in the Drosophila brain. They signal via the neuropeptide pigment‐dispersing factor (PDF) to align the molecular clockwork of different central clock neurons and to modulate downstream circuits. The dorsal terminals of the sLNvs undergo daily morphological changes that affect presynaptic sites organised by the active zone protein Bruchpilot (BRP), a homolog of mammalian ELKS proteins. However, the role of these presynaptic sites for PDF release is ill‐defined.Here, we combined expansion microscopy with labelling of active zones by endogenously tagged BRP to examine the spatial correlation between PDF‐containing dense‐core vesicles and BRP‐labelled active zones. We found that the number of BRP‐labelled puncta in the sLNv terminals was similar while their density differed between Zeitgeber time (ZT) 2 and 14. The relative distance between BRP‐ and PDF‐labelled puncta was increased in the morning, around the reported time of PDF release. Spontaneous dense‐core vesicle release profiles of sLNvs in a publicly available ssTEM dataset (FAFB) consistently lacked spatial correlation to BRP‐organised active zones. RNAi‐mediated downregulation of brp and other active zone proteins expressed by the sLNvs did not affect PDF‐dependent locomotor rhythmicity. In contrast, down‐regulation of genes encoding proteins of the canonical vesicle release machinery, the dense‐core vesicle‐related protein CADPS, as well as PDF impaired locomotor rhythmicity.Taken together, our study suggests that PDF release from the sLNvs is independent of BRP‐organised active zones, while BRP may be redistributed to active zones in a time‐dependent manner.