GLABROUS INFLORESCENCE STEMS3 binds to and activates RHD2 and RHD4 genes to promote root hair elongation in Arabidopsis

Author:

Huang Linli12,Xu Nuo1,Wu Junyu1,Yang Shuaiqi1,An Lijun3ORCID,Zhou Zhongjing4,Wong Chui Eng5,Wu Mingjie1,Yu Hao5ORCID,Gan Yinbo1ORCID

Affiliation:

1. Department of Agronomy, College of Agriculture and Biotechnology Zhejiang University Hangzhou China

2. Biotechnology Research Institute, Shanghai Academy of Agricultural Science Shanghai Key Laboratory of Agricultural Genetics and Breeding Shanghai China

3. College of Life Sciences Northwest A&F University Shanxi China

4. State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro‐Products Zhejiang Academy of Agricultural Sciences Hangzhou China

5. Department of Biological Sciences and Temasek Life Sciences Laboratory National University of Singapore Singapore

Abstract

SUMMARYRoot hairs are crucial in the uptake of essential nutrients and water in plants. This study showed that a zinc finger protein, GIS3 is involved in root hair growth in Arabidopsis. The loss‐of‐function gis3 and GIS3 RNAi transgenic line exhibited a significant reduction in root hairs compared to the wild type. The application of 1‐aminocyclopropane‐1‐carboxylic acid (ACC), an exogenous ethylene precursor, and 6‐benzyl amino purine (BA), a synthetic cytokinin, significantly restored the percentage of hair cells in the epidermis in gis3 and induced GIS3 expression in the wild type. More importantly, molecular and genetic studies revealed that GIS3 acts upstream of ROOT HAIR DEFECTIVE 2 (RHD2) and RHD4 by binding to their promoters. Furthermore, exogenous ACC and BA application significantly induced the expression of RHD2 and RHD4, while root hair phenotype of rhd2‐1, rhd4‐1, and rhd4‐3 was insensitive to ACC and BA treatment. We can therefore conclude that GIS3 modulates root hair development by directly regulating RHD2 and RHD4 expression through ethylene and cytokinin signals in Arabidopsis.

Funder

National Natural Science Foundation of China

Publisher

Wiley

Subject

Cell Biology,Plant Science,Genetics

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