CLEM, a universal tool for analyzing structural organization in thylakoid membranes

Author:

Lübben Maximilian K.1,Klingl Andreas2,Nickelsen Jörg1,Ostermeier Matthias1ORCID

Affiliation:

1. Department of Molecular Plant Science LMU Munich Planegg‐Martinsried Germany

2. Plant Development LMU Munich Planegg‐Martinsried Germany

Abstract

AbstractChlorophyll (Chl) plays a crucial role in photosynthesis, functioning as a photosensitizer. As an integral component of this process, energy absorbed by this pigment is partly emitted as red fluorescence. This signal can be readily imaged by fluorescence microscopy and provides a visualization of photosynthetic activity. However, due to limited resolution, signals cannot be assigned to specific subcellular/organellar membrane structures. By correlating fluorescence micrographs with transmission electron microscopy, researchers can identify sub‐cellular compartments and membranes, enabling the monitoring of Chl distribution within thylakoid membrane substructures in cyanobacteria, algae, and higher plant single cells. Here, we describe a simple and effective protocol for correlative light‐electron microscopy (CLEM) based on the autofluorescence of Chl and demonstrate its application to selected photosynthetic model organisms. Our findings illustrate the potential of this technique to identify areas of high Chl concentration and photochemical activity, such as grana regions in vascular plants, by mapping stacked thylakoids.

Funder

Deutsche Forschungsgemeinschaft

Publisher

Wiley

Cited by 1 articles. 订阅此论文施引文献 订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献

1. From Photosynthesis to Industrial Applications;Physiologia Plantarum;2024-07

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