High‐throughput sequencing revealed low‐efficacy genome editing using Cas9 <scp>RNPs</scp> electroporation and single‐celled microinjection provided an alternative to deliver <scp>CRISPR</scp> reagents into <i>Euglena gracilis</i>-Reference-Cited by-同舟云学术

High‐throughput sequencing revealed low‐efficacy genome editing using Cas9 RNPs electroporation and single‐celled microinjection provided an alternative to deliver CRISPR reagents into Euglena gracilis

Published:2022-09-15 Issue:11 Volume:20 Page:2048-2050
ISSN:1467-7644
Container-title:Plant Biotechnology Journal
language:en
Short-container-title:Plant Biotechnology Journal

Author:

Chen Zhenfan¹^ORCID,Zhu Jiayi¹,Chen Zixi¹,Du Ming¹,Yao Rao¹,Fu Wen¹,Lei Anping¹,Wang Jiangxin¹^ORCID

Affiliation:

1. Shenzhen Key Laboratory of Marine Bioresource and Eco‐Environmental Science, Shenzhen Engineering Laboratory for Marine Algal Biotechnology, Guangdong Provincial Key Laboratory for Plant Epigenetics, College of Life Sciences and Oceanography Shenzhen University Shenzhen China

Funder

National Key Research and Development Program of China

National Natural Science Foundation of China

Publisher

Wiley

Subject

Plant Science,Agronomy and Crop Science,Biotechnology

Link

https://onlinelibrary.wiley.com/doi/pdf/10.1111/pbi.13915

Reference8 articles.

1. A Synthetic Biology Perspective on the Bioengineering Tools for an Industrial Microalga: Euglena gracilis

2. Genetic transformation of mouse embryos by microinjection of purified DNA.