Affiliation:
1. Department of Orthodontics Affiliated Stomatological Hospital Fujian Medical University Fuzhou China
2. Department of Stomatology The Second Affiliated Hospital of FMU Quanzhou China
Abstract
AbstractObjectiveThis study aimed to evaluate the role of miR‐150‐5p in the onset and progression of periodontitis, and reveal the potential molecular mechanism underlying its function and to explore a novel biomarker for periodontitis.BackgroundPeriodontitis is the leading cause of tooth loss in adults, emphasizing the need for a biomarker to improve its early detection and prevention. The association of miR‐150‐5p with diseases related to Fuscobacterium nucleatum implies its potential involvement in periodontitis.MethodsThe expression of miR‐150‐5p in the saliva of patients with periodontitis (n = 77) and healthy individuals (n = 43) was assessed by PCR. Human gingival fibroblasts (HGFs) were induced with an osteogenic culture medium. The regulatory effect of miR‐150‐5p on the proliferation and migration of HGFs was assessed by CCK8 and transwell assays. Osteogenic differentiation was estimated based on the expression of corresponding factors through western blotting, and the inflammatory response was evaluated by measuring the levels of pro‐inflammatory cytokines using ELISA.ResultsSignificant upregulation of miR‐150‐5p was observed in patients with periodontitis, which sensitively distinguished them and was closely associated with the severity and periodontal index of the condition. In HGFs, osteogenic induction (OI) resulted in increased miR‐150‐5p levels, which negatively regulated the expression of AIFM2. Silencing miR‐150‐5p significantly attenuated OI‐induced suppression of proliferation and migration of HGFs. The silencing also alleviated inflammation and osteogenic differentiation, which was reversed upon AIFM2 knockdown.ConclusionUpregulated miR‐150‐5p in periodontitis served as a diagnostic biomarker, indicating the occurrence and aggravation of disease condition. Silencing miR‐150‐5p inhibited the osteogenic differentiation and inflammation of HGFs by negatively modulating AIFM2.
Reference37 articles.
1. Periodontitis: facts, fallacies and the future;Slots J;Periodontol 2000,2017
2. Focal infection of periodontal origin;Slots J;Periodontol 2000,2019
3. Periodontitis and cardiovascular diseases: consensus report;Sanz M;J Clin Periodontol,2020
4. Periodontitis and diabetes: a two‐way relationship;Preshaw PM;Diabetologia,2012
5. Association between chronic periodontitis and rheumatoid arthritis. A systematic review;Wen S;Rev Med Chil,2019