The need to freeze—Dehydration during specimen preparation for electron microscopy collapses the endothelial glycocalyx regardless of fixation method

Author:

Hempel Casper123ORCID,Kapishnikov Sergey45ORCID,Perez‐Berna Ana Joaquina6,Werner Stephan4,Guttmann Peter4ORCID,Pereiro Eva6ORCID,Qvortrup Klaus7ORCID,Andresen Thomas Lars1ORCID

Affiliation:

1. Department of Health Technology Technical University of Denmark Lyngby Denmark

2. Centre for Medical Parasitology Department for Clinical Microbiology Copenhagen University Hospital Copenhagen Denmark

3. Department for Immunology and Microbiology University of Copenhagen Copenhagen Denmark

4. Department X‐Ray microscopy Helmholtz‐Zentrum Berlin für Materialien und Energie GmbH Berlin Germany

5. Niels Bohr Institute University of Copenhagen Copenhagen Denmark

6. MISTRAL Beamline‐Experiments Division ALBA Synchrotron Light Source Barcelona Spain

7. Core Facility for Integrated Microscopy (CFIM) University of Copenhagen Copenhagen Denmark

Funder

Carlsbergfondet

European Molecular Biology Organization

Fonden til Lægevidenskabens Fremme

Det Frie Forskningsråd

Lundbeckfonden

Rigshospitalet

Publisher

Wiley

Subject

Physiology (medical),Cardiology and Cardiovascular Medicine,Molecular Biology,Physiology

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