Novel method utilizing bisulfite conversion with dual amplification‐refractory mutation system polymerase chain reaction to detect circulating pancreatic β‐cell cfDNA

Author:

Okada Asami1,Yamada‐Yamashita Misuzu2,Tominaga Yukari2,Jo Kyoka2,Mori Hiroyasu2,Suzuki Reiko2,Ishizu Masashi2,Tamaki Motoyuki2,Akehi Yuko2,Takashi Yuichi2ORCID,Koga Daisuke3,Shimokita Eisuke4,Tanihara Fuminori5,Kurahashi Kiyoe6,Yoshida Sumiko6,Mitsui Yukari6,Masuda Shiho6,Endo Itsuro67,Aihara Ken‐ichi68ORCID,Kagami Shoji1,Abe Masahiro6,Ferreri Kevin9,Fujitani Yoshio10ORCID,Matsuhisa Munehide2ORCID,Kuroda Akio2ORCID

Affiliation:

1. Department of Pediatrics Tokushima University Tokushima Japan

2. Diabetes Therapeutics and Research Center Institute of Advanced Medical Sciences Tokushima University Tokushima Japan

3. Diagnostic Division, Department of Research Otsuka Pharmaceutical Co., Ltd. Tokushima Japan

4. Department of Anatomy and Cell Biology Tokushima University Graduate School of Medical Sciences Tokushima Japan

5. Animal Resource Laboratory Center for Development of Advanced Medical Technology Jichi Medical University Shimotsuke Japan

6. Department of Hematology, Endocrinology and Metabolism Tokushima University Graduate School of Biomedical Sciences Tokushima Japan

7. Department of Chronomedicine Tokushima University Graduate School of Medical Sciences Tokushima Japan

8. Department of Community Medicine and Medical Science Tokushima University Graduate School of Biomedical Sciences Tokushima Japan

9. Department of Translational Research & Cellular Therapeutics City of Hope Duarte California USA

10. Developmental Biology and Metabolism Institute for Molecular and Cellular Regulation Gunma University Maebashi Japan

Publisher

Wiley

Subject

General Medicine,Endocrinology, Diabetes and Metabolism,Internal Medicine

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