Do diverse wheat genotypes unleash their biochemical arsenal differentially to conquer cold stress? A comprehensive study in the Western Himalayas

Author:

Jan Sofora1,Kumar Sandeep1,Yousuf Munaza1,Shafi Safoora1,Majid Ronak1,Khan M. Anwar1,Jeelani Fehim2,Shikari Asif Bashir1,Kaur Satinder3,Kumar Sundeep4,Kalia Sanjay5,Singh Kuldeep6,Prasad Manoj78ORCID,Varshney Rajeev K.9ORCID,Mir Reyazul Rouf1ORCID

Affiliation:

1. Division of Genetics & Plant Breeding Faculty of Agriculture (FoA) Sopore Kashmir, J&K India

2. Division of Agricultural Economics & Statistics Wadura India

3. School of Agricultural Biotechnology Punjab Agricultural University Ludhiana Punjab India

4. Division of Genomic Resources NBPGR New Delhi India

5. Department of Biotechnology (DBT) Government of India New Delhi India

6. Gene Bank/Accelerated Crop Improvement Program ICRISAT Patancheru Telangana India

7. National Institute of Plant Genome Research New‐Dehli India

8. Department of Genetics University of Delhi, South Campus, Dhaula Kuan New Delhi India

9. Centre for Crop & Food Innovation State Agricultural Biotechnology Centre, Food Futures Institute, Murdoch University Murdoch WA Australia

Abstract

AbstractWheat is one of the most important cereal crops in the world. Cold stress is a major constraint in production of wheat grown in cold climate regions. In this study, we conducted a comprehensive assessment of cold stress tolerance in wheat genotypes through field screening, cell membrane stability through electrolyte leakage assay and biochemical profiling. A core set comprising 4560 genotypes was evaluated for two years (2021–2022), revealing substantial genetic variation for cold stress tolerance. Most genotypes exhibited moderate tolerance, while a smaller proportion showed susceptibility to cold stress. Based on the cold screening data in the field, a mini‐core set of 350 genotypes was selected for membrane stability analysis using electrical conductivity assays. Significant differences were observed in membrane stability among the genotypes, indicating the presence of genetic variation for this trait. Furthermore, a mini‐core set was narrowed down to 50 diverse candidate genotypes that were subsequently profiled for various biochemicals, including reactive oxygen species (ROS) like lipid peroxidation (MDA) and hydrogen peroxide (H202), osmoprotectant (proline) and enzymatic antioxidants including ascorbate peroxidase (APX), superoxide dismutase (SOD), guaiacol peroxidase (GPX), and catalase (CAT). Correlation analysis of the biochemicals revealed negative associations between antioxidants and reactive oxygen species (ROS), highlighting their role in mitigating oxidative damage under cold stress. This study enhances our understanding of the physiological and biochemical mechanisms underlying cold stress tolerance in wheat. The identified genotypes with superior cold stress tolerance can serve as valuable genetic resources for wheat breeding.

Funder

Department of Biotechnology, Ministry of Science and Technology, India

Publisher

Wiley

Subject

Cell Biology,Plant Science,Genetics,General Medicine,Physiology

Reference61 articles.

1. Effect of cold stress on polyamine metabolism and antioxidant responses in chickpea

2. Response of antioxidative defense system to low temperature stress in two wheat cultivars;Apostolova P.;General and Applied Plant Physiology,2008

3. Recent advances in abiotic stress tolerance of plants through chemical priming: an overview;Ashraf M. A.;Advances in seed priming,2018

4. The Role of Non-Enzymatic Antioxidants in Improving Abiotic Stress Tolerance in Plants

5. Temperature stress and redox homeostasis in agricultural crops

Cited by 2 articles. 订阅此论文施引文献 订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3