Affiliation:
1. Department of Neurosurgery General Hospital of Northern Theater Command Shenyang China
2. Department of Neurology General Hospital of Northern Theater Command Shenyang China
Abstract
AbstractActivation and polarization of microglia play decisive roles in the progression of intracerebral haemorrhage (ICH), and lactate exposure correlates with microglia polarization. This study explores molecules influencing lactate production and microglia phenotype alteration following ICH. A murine model of ICH was induced by intracerebral injection of collagenase. The mice experienced autonomous neurological function recovery, haematoma resolution and rapid lactate production, along with a gradual increase in angiogenesis activity, neuronal recovery and an M1‐to‐M2 phenotype change of microglia. Galloflavin, a lactate dehydrogenase antagonist, suppressed this phenotype change and the functional recovery in mice. FOS like 2 (FOSL2) was significantly upregulated in the brain tissues from day 7 post‐ICH. Overexpression of FOSL2 induced an M1‐to‐M2 phenotype shift in microglia and accelerated lactate production in vivo and in haemoglobin‐treated microglia in vitro. Long non‐coding RNA MIR17HG impeded FOSL2‐mediated transcription activation of hypermethylated in cancer 1 (HIC1). MIR17HG overexpression induced pro‐inflammatory activation of microglia in mice, which was blocked by further HIC1 overexpression. Overall, this study demonstrates that MIR17HG maintains a pro‐inflammatory phenotype of microglia during ICH progression by negating FOSL2‐mediated transcription activation of HIC1. Specific inhibition of MIR17HG or upregulation of FOSL2 or HIC1 may favour inflammation inhibition and haematoma resolution in ICH.