Simultaneous measurement of quantum yield ratio and absorption ratio between acceptor and donor by linearly unmixing excitation-emission spectra
Author:
Affiliation:
1. MOE Key Laboratory of Laser Life Science & College of Life Science; South China Normal University; Guangzhou China
2. Key Laboratory of Optoelectronic Devices; Shenzhen University; Shenzhen China
Funder
National Natural Science Foundation of China
Publisher
Wiley
Subject
Histology,Pathology and Forensic Medicine
Link
http://onlinelibrary.wiley.com/wol1/doi/10.1111/jmi.12687/fullpdf
Reference20 articles.
1. Detecting stoichiometry of macromolecular complexes in live cells using FRET;Ben-Johny;Nat. Commun.,2016
2. Quantifying macromolecular interactions in living cells using FRET two-hybrid assays;Butz;Nat. Protoc.,2016
3. Miniature fiber optic spectrometer-based quantitative fluorescence resonance energy transfer measurement in single living cells;Chai;J. Biomed. Opt.,2015
4. Measurement of FRET efficiency and ratio of donor to acceptor concentration in living cells;Chen;Biophys. J.,2006
5. Multichannel wide-field microscopic FRET imaging based on simultaneous spectral unmixing of excitation and emission spectra;Du;J. Microsc.,2017
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3. Quantitative Fluorescence Resonance Energy Transfer Measurement Based on Spectral Unmixing;CHIN J LASERS;2020
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