Serum concentrations of secretory leukocyte protease inhibitor and IL6 can predict the onset of sepsis in pyometra bitches

Author:

Sasidharan Jisna Kuttiyeri1,Patra Manas Kumar1ORCID,De Ujjwal Kumar2,Khan Javid Ahmad1,Shah Irfan1,Mathesh Karikalan3,Saxena Abhishek Chandra4,Dubal Zunjar Baburao5,Singh Sanjay Kumar1,Kumar Harendra1,Krishnaswamy Narayanan6ORCID

Affiliation:

1. Animal Reproduction Division Indian Council of Agricultural Research (ICAR)‐Indian Veterinary Research Institute (IVRI) Izatnagar India

2. Division of Medicine Indian Council of Agricultural Research (ICAR)‐Indian Veterinary Research Institute (IVRI) Izatnagar India

3. Centre for Wildlife Conservation, Management and Disease Surveillance Indian Council of Agricultural Research (ICAR)‐Indian Veterinary Research Institute (IVRI) Izatnagar India

4. Division of Surgery Indian Council of Agricultural Research (ICAR)‐Indian Veterinary Research Institute (IVRI) Izatnagar India

5. Division of Veterinary Public Health Indian Council of Agricultural Research (ICAR)‐Indian Veterinary Research Institute (IVRI) Izatnagar India

6. Foot and mouth disease research laboratory Indian Council of Agricultural Research (ICAR)‐Indian Veterinary Research Institute (IVRI) Hebbal, Bengaluru India

Abstract

AbstractAs onset of sepsis adversely affects the prognosis of canine pyometra, finding biomarkers that would distinguish sepsis status would be useful in the clinical management. Accordingly, we hypothesized that differential expression of endometrial transcripts and circulating concentration of certain inflammatory mediators would discriminate pyometra‐led sepsis (P‐sepsis+) from those of pyometra without sepsis (P‐sepsis−). Bitches with pyometra (n = 52) were classified into P‐sepsis+ (n = 28) and P‐sepsis− (n = 24) based on vital clinical score and total leukocyte count. A group of non‐pyometra bitches (n = 12) served as control. The relative fold changes in the transcripts of IL6, IL8, TNFα, IL10, PTGS2, mPGES1 and PGFS, SLPI, S100A8, S100A12 and eNOS were determined by quantitative polymerase chain reaction. Furthermore, the serum concentrations of IL6, IL8, IL10, SLPI and prostaglandin F metabolite (PGFM) were assayed by ELISA. The relative fold changes in S100A12 and SLPI and mean concentrations of IL6 and SLPI were significantly (p < .05) higher in P‐sepsis+ than that of P‐sepsis− group. Receiver operating characteristic analysis revealed that serum IL6 had a diagnostic sensitivity of 78.6% and a positive likelihood ratio (LR+) of 2.09, at a cut‐off value of 15.7 pg/mL to diagnose P‐sepsis+ cases. Similarly, serum SLPI had a sensitivity of 84.6% and an LR+ of 2.23, at a cut‐off value of 2.0 pg/mL. It was concluded that SLPI and IL6 would serve as putative biomarkers for pyometra‐led sepsis in bitches. Monitoring SLPI and IL6 would be a useful adjunct to the established haemato‐biochemical parameters in customizing the treatment strategies and arriving at the decision for management of pyometra bitches with critical illness.

Funder

Department of Biotechnology, Government of West Bengal

Publisher

Wiley

Subject

Endocrinology,Animal Science and Zoology,Biotechnology

Reference46 articles.

1. Stress molecules in sepsis and systemic inflammatory response syndrome

2. Comparison of clinical and inflammatory parameters in dogs with pyometra before and after ovariohysterectomy;Ahn S.;Canadian Journal of Veterinary Research,2021

3. Cytokine profiles as markers of disease severity in sepsis: a multiplex analysis

4. Cytokine release in sepsis

5. Role of cytokines as a double‐edged sword in sepsis;Chaudhry H.;In Vivo,2013

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