Affiliation:
1. Department of Clinical Laboratory The Second Hospital of Jilin University Changchun China
2. Department of Emergency and Critical Care Medicine The Second Hospital of Jilin University Changchun China
3. Eye Center The Second Hospital of Jilin University Changchun China
4. Jilin Provincial Key Laboratory on Molecular and Chemical Genetics The Second Hospital of Jilin University Changchun China
Abstract
This study was performed to explore the antifungal and antibiofilm effects of polyphyllin I (PPI) on Candida albicans. Microdilution assay was performed to determine the minimal inhibitory concentrations (MIC) of PPI against Candida species. Adhesion assay, hyphal growth assay, biofilm formation, and development were used to test the impacts of PPI on C. albicans virulence factors. Propidium iodide staining was performed to test whether the permeability of cell membrane was influenced by PPI. PPI showed significant antifungal activities against several Candida species, with MIC below or equal to 6.25 μM. PPI also inhibited the adhesion to polystyrene surfaces, hyphal growth, and biofilm formation. PPI significantly increased the permeability of C. albicans cell membrane. In sum, PPI can suppress the planktonic growth and biofilm of C. albicans and its mechanism involves the increased permeability of cell membrane.
Funder
Natural Science Foundation of Jilin Province
Subject
Microbiology (medical),General Medicine,Immunology and Allergy,Pathology and Forensic Medicine
Cited by
1 articles.
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