Cryo-Immunoelectron Microscopy of Adherent Cells Improved by the Use of Electrospun Cell Culture Substrates

Author:

Schmiedinger Thomas1,Vogel Georg F.,Eiter Oliver1,Pfaller Kristian2,Kaufmann Walter A.3,Flörl Angelika2,Gutleben Karin2,Schönherr Sabine3,Witting Barbara2,Lechleitner Thomas W.1,Ebner Hannes-L.,Seppi Thomas1,Hess Michael W.2

Affiliation:

1. Department of Therapeutic Radiology and Oncology; Innsbruck Medical University; Anichstrasse 35; A-6020; Innsbruck; Austria

2. Division of Histology and Embryology; Innsbruck Medical University; Müllerstrasse 59; A-6020; Innsbruck; Austria

3. Institute of Pharmacology; Innsbruck Medical University; Peter Mayr Strasse 1a; A-6020; Innsbruck; Austria

Publisher

Wiley

Subject

Cell Biology,Genetics,Molecular Biology,Biochemistry,Structural Biology

Reference49 articles.

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2. Immunogold labeling of cryosections from high-pressure frozen cells;Donselaar;Traffic,2007

3. Cryo-section immunolabelling of difficult to preserve specimens: advantages of cryofixation, freeze-substitution and rehydration;Ripper;Biol Cell Eur Cell Biol Organ,2008

4. A technique for ultracryotomy of cell suspensions and tissues;Tokuyasu;J Cell Biol,1973

5. Clathrin-mediated constitutive endocytosis of PIN auxin efflux carriers in Arabidopsis;Dhonukshe;Curr Biol,2007

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