lncRNA HOXA11‐AS maintains the stemness of oral squamous cell carcinoma stem cells and reduces the radiosensitivity by targeting miR‐518a‐3p/PDK1

Author:

Li Baojun1,Lv Yuanjing1,Zhang Cui2,Xiang Cheng1

Affiliation:

1. Department of Head and Neck Surgery Harbin Medical University Cancer Hospital Harbin Heilongjiang China

2. Department of Medical Ultrasound Harbin Medical University Cancer Hospital Harbin Heilongjiang China

Abstract

AbstractObjectiveOral squamous cell carcinoma (OSCC) is the most prevailing oral malignancy. The lncRNA HOXA11‐AS shows prominent roles in OSCC. This study explored the effects of lncRNA HOXA11‐AS on regulating OSCC stem cell stemness and radiosensitivity by targeting miR‐518a‐3p/PDK1.MethodsHuman OSCC cell lines SCC9 and SCC15 were selected. CD133+ cancer stem cells (CSCs) were sorted by immunomagnetic beads. CD133 expression in cells and HOXA11‐AS expression in SCC9, SCC15, and CD133+ SCC9, CD133+ SCC15 cells were assessed by flow cytometry and RT‐qPCR. HOXA11‐AS was silenced/overexpressed in SCC9, SCC15, CD133+ SCC9, and CD133+ SCC15 cells. Cell proliferation, radiosensitivity, invasion, and stem cell sphere formation ability were examined by CCK‐8, colony formation, Transwell, and stem cell sphere formation. The levels of stemness‐related genes (Oct4, Nanog, Sox2), miR‐518a‐3p, epithelial–mesenchymal transition (EMT)‐related proteins (E‐cadherin, Vimentin, N‐cadherin), and PDK1 were assessed by RT‐qPCR and Western blot assay.ResultsHOXA11‐AS was up‐regulated in SCC9, SCC15, CD133+ SCC9, and CD133+ SCC15 cells. HOXA11‐AS silencing inhibited OSCC proliferation and invasion and enhanced radiosensitivity. HOXA11‐AS maintained CSC stemness in OSCC. HOXA11‐AS silencing reduced CD133+ SCC9 and CD133+ SCC15 stem cell sphere formation ability, reduced stem cell stemness‐related gene levels, and inhibited EMT. HOXA11‐AS regulated OSCC stem cell stemness and radiosensitivity by targeting miR‐518a‐3p. PDK1 overexpression annulled the regulatory effects of HOXA11‐AS silencing on OSCC cell stem cell stemness and radiosensitivity.ConclusionIn vitro lncRNA HOXA11‐AS silencing inhibited OSCC stem cell stemness by targeting the miR‐518a‐3p/PDK1 axis, thus enhancing OSCC cell radiosensitivity.

Publisher

Wiley

Subject

Periodontics,Cancer Research,Otorhinolaryngology,Oral Surgery,Pathology and Forensic Medicine

Cited by 4 articles. 订阅此论文施引文献 订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3