Affiliation:
1. Agricultural Biotechnology and Chemical Ecology (ABCE) Lab Indian Institute of Science Education and Research Pune Maharashtra 411008 India
2. Division of Crop Protection ICAR Research Complex for NEH Region Umiam Meghalaya 793103 India
3. ICAR‐Directorate of Floricultural Research Pune Maharashtra 411036 India
Abstract
Summary
Eggplant (Solanum melongena) suffers severe losses due to a multi‐insecticide‐resistant lepidopteran pest, shoot and fruit borer (SFB, Leucinodes orbonalis). Heavy and combinatorial application of pesticides for SFB control renders eggplant risky for human consumption. We observed that gravid SFB females do not oviposit on Himalayan eggplant variety RC‐RL‐22 (RL22). We hypothesized that RL22 contained an antixenosis factor.
Females' behavior indicated that the RL22 cue they perceived was olfactory. To identify it, leaf volatile blends of seven eggplant varieties were profiled using solid phase microextraction and gas chromatography mass spectrometry.
Seven RL22‐specific compounds were detected in the plant headspace. In choice assays, oviposition deterrence efficacies of these candidate compounds were independently tested by their foliar application on SFB‐susceptible varieties. Complementation of geraniol, which was exclusively found in RL22, reduced oviposition (> 90%). To validate geraniol's role in RL22's SFB‐deterrence, we characterized RL22's geraniol synthase and silenced its gene in planta, using virus‐induced gene silencing. Geraniol biosynthesis suppression rendered RL22 SFB‐susceptible; foliar geraniol application on the geraniol synthase‐silenced plants restored oviposition deterrence.
We infer that geraniol is RL22's SFB oviposition deterrent. The use of natural compounds like geraniol, which influence the chemical ecology of oviposition, can reduce the load of hazardous synthetic larvicides.
Funder
Council of Scientific and Industrial Research, India
Indian Institute of Science Education and Research Pune
Ministry of Human Resource Development
Science and Engineering Research Board
Cited by
1 articles.
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