Safety profile and effects on the peripheral immune response of fecal microbiota transplantation in clinically healthy dogs

Author:

Lee Mary Ann1ORCID,Questa Maria1,Wanakumjorn Patrawin2,Kol Amir2,McLaughlin Bridget3,Weimer Bart C.4,Buono Agostino5,Suchodolski Jan S.5ORCID,Marsilio Sina1ORCID

Affiliation:

1. Department of Medicine and Epidemiology University of California School of Veterinary Medicine, University of California Davis California USA

2. Department of Pathology, Microbiology & Immunology School of Veterinary Medicine, University of California Davis California USA

3. Flow Cytometry Shared Resource Laboratory University of California, Davis Davis California USA

4. Department of Population Health and Reproduction, 100K Pathogen Genome Project University of California School of Veterinary Medicine, University of California Davis California USA

5. Gastrointestinal Laboratory Texas A&M School of Veterinary Medicine & Biomedical Sciences College Station Texas USA

Abstract

AbstractBackgroundFecal microbiota transplantation (FMT) is increasingly used for gastrointestinal and extra‐gastrointestinal diseases in veterinary medicine. However, its effects on immune responses and possible adverse events have not been systematically investigated.Hypothesis/ObjectivesDetermine the short‐term safety profile and changes in the peripheral immune system after a single FMT administration in healthy dogs.AnimalsTen client‐owned, clinically healthy dogs as FMT recipients, and 2 client‐owned clinically healthy dogs as FMT donors.MethodsProspective non‐randomized clinical trial. A single rectal enema of 5 g/kg was given to clinically healthy canine recipients. During the 28 days after FMT administration, owners self‐reported adverse events and fecal scores. On Days 0 (baseline), 1, 4, 10, and 28 after FMT, fecal and blood samples were collected. The canine fecal dysbiosis index (DI) was calculated using qPCR.ResultsNo significant changes were found in the following variables: CBC, serum biochemistry, C‐reactive protein, serum cytokines (interleukins [IL]‐2, ‐6, ‐8, tumor necrosis factor [TNF]‐α), peripheral leukocytes (B cells, T cells, cluster of differentiation [CD]4+ T cells, CD8+ T cells, T regulatory cells), and the canine DI. Mild vomiting (n = 3), diarrhea (n = 4), decreased activity (n = 2), and inappetence (n = 1) were reported, and resolved without intervention.Conclusions and Clinical ImportanceFecal microbiota transplantation did not significantly alter the evaluated variables and recipients experienced minimal adverse events associated with FMT administration. Fecal microbiota transplantation was not associated with serious adverse events, changes in peripheral immunologic variables, or the canine DI in the short‐term.

Funder

Comparative Gastroenterology Society

Comprehensive Cancer Center, City of Hope

Publisher

Wiley

Cited by 1 articles. 订阅此论文施引文献 订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3