Affiliation:
1. Department of Medicine and Epidemiology University of California School of Veterinary Medicine, University of California Davis California USA
2. Department of Pathology, Microbiology & Immunology School of Veterinary Medicine, University of California Davis California USA
3. Flow Cytometry Shared Resource Laboratory University of California, Davis Davis California USA
4. Department of Population Health and Reproduction, 100K Pathogen Genome Project University of California School of Veterinary Medicine, University of California Davis California USA
5. Gastrointestinal Laboratory Texas A&M School of Veterinary Medicine & Biomedical Sciences College Station Texas USA
Abstract
AbstractBackgroundFecal microbiota transplantation (FMT) is increasingly used for gastrointestinal and extra‐gastrointestinal diseases in veterinary medicine. However, its effects on immune responses and possible adverse events have not been systematically investigated.Hypothesis/ObjectivesDetermine the short‐term safety profile and changes in the peripheral immune system after a single FMT administration in healthy dogs.AnimalsTen client‐owned, clinically healthy dogs as FMT recipients, and 2 client‐owned clinically healthy dogs as FMT donors.MethodsProspective non‐randomized clinical trial. A single rectal enema of 5 g/kg was given to clinically healthy canine recipients. During the 28 days after FMT administration, owners self‐reported adverse events and fecal scores. On Days 0 (baseline), 1, 4, 10, and 28 after FMT, fecal and blood samples were collected. The canine fecal dysbiosis index (DI) was calculated using qPCR.ResultsNo significant changes were found in the following variables: CBC, serum biochemistry, C‐reactive protein, serum cytokines (interleukins [IL]‐2, ‐6, ‐8, tumor necrosis factor [TNF]‐α), peripheral leukocytes (B cells, T cells, cluster of differentiation [CD]4+ T cells, CD8+ T cells, T regulatory cells), and the canine DI. Mild vomiting (n = 3), diarrhea (n = 4), decreased activity (n = 2), and inappetence (n = 1) were reported, and resolved without intervention.Conclusions and Clinical ImportanceFecal microbiota transplantation did not significantly alter the evaluated variables and recipients experienced minimal adverse events associated with FMT administration. Fecal microbiota transplantation was not associated with serious adverse events, changes in peripheral immunologic variables, or the canine DI in the short‐term.
Funder
Comparative Gastroenterology Society
Comprehensive Cancer Center, City of Hope
Cited by
1 articles.
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