Induction of CD8+ immune memory and enhanced inflammation in a skin inflammation model through pre‐immunization with inactivated pathogens

Author:

Falkenberg Caroline1ORCID,Bartholdy Christina2ORCID,Koch Janne2,Toft Martin Fitzner3ORCID,Skov Søren1ORCID,Hansen Camilla Hartmann Friis1ORCID,Hansen Axel Kornerup1ORCID

Affiliation:

1. Department of Veterinary and Animal Sciences, Faculty of Health and Medical Sciences University of Copenhagen Frederiksberg C Denmark

2. Translational Sciences, Research & Early Development, LEO Pharma A/S Ballerup Denmark

3. QM Diagnostics Nijmegen The Netherlands

Abstract

AbstractLaboratory mice live in specific pathogen‐free (SPF) conditions, resulting in an immature immune system comparable to that of newborns rather than adult humans or mice from pet shops. This condition may compromise their translational value. Reintroducing pathogens would lead to the uncontrolled spread of infections and associated diseases, so research facilities should seek safer alternatives. We immunized laboratory mice with a cocktail of pathogens, which were inactivated by ultraviolet irradiation and mixed with the adjuvant AddaVax. This immunization resulted in a higher percentage of CD8+ effector memory T cells compared to untreated mice, although the response was not as robust as in pet shop mice. In a model of skin inflammation, pre‐immunization led to an increased skin inflammatory response compared to non‐immunized mice. All immunized mice seroconverted to the pathogens in the mixture, while none of the non‐immunized mice housed together seroconverted to the pathogens applied to the pre‐immunized mice. In conclusion, pre‐immunization of mice impacts the immune system, which includes increasing the levels of CD8+ effector memory T cells.

Publisher

Wiley

Subject

General Pharmacology, Toxicology and Pharmaceutics,General Biochemistry, Genetics and Molecular Biology,General Medicine,General Neuroscience

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