Adhesion of peripheral blood lymphocytes of children with arthritis to human umbilical vein endothelial cells

Abstract

SUMMARY To determine whether adhesion of peripheral blood lymphocytes (PBL) of patients with juvenile rheumatoid arthritis (JRA) may be enhanced, adhesion of PBL of children with JRA. children with seronegative spondyloarthropathies (SSA). age-appropriate and adult controls, to human umbilical vein endothelial cells (HUVEC) was assessed in vitro. B and CD4 T lymphocytes in initial, adherent, and non-adherent cell fractions were identified by flow cytometry. B lymphocytes of all the younger subjects combined had a higher adherence to activated HUVEC compared with B lymphocytes of the adult donors. Except for greater adherence of HLA-DR+ CD4 T cells, lymphocytes of children with JRA showed no enhanced adhesion to either unactivated or activated HUVEC. The percentage of B cells adherent to activated HUVEC in each of the subject groups was 1-5 3-6-fold higher than adherent CD4 T lymphocytes. Surface analyses indicated higher percentages of CD49d (α4)+ and CD29 (β1)+ CD4 T lymphocytes in adherent cells, but less of a differential in CD49 (α4)+ and no difterence in CD29(βl)+ B lymphocytes. There were fewer Leu-8(L-selectin)+ B and Leu-8+ CD4T cells among adherent cells. The data suggest a greater adhesive capacity of B lymphocytes compared with CD4 T lymphocytes which is unrelated to disease, and the possibility that B lymphocytes may utilize adhesion molecules distinct from those of CD4 T lymphocytes. Only a small subset of T cells of patients with JRA may have an enhanced capacity for adhesion to endothelium.