Affiliation:
1. Internal Medicine I, National Defence Medical College, Tokorozawa, Saitama, Japan
Abstract
SUMMARY
Lipid microspheres (LM) used as drug carriers increase the effectiveness and reduce the toxicity of incorporated drugs. The present study is designed to determine whether or not activated T lymphocytes, which were the cells chosen first from the ‘inflammatory cells’, can take up LM in vitro. LM were labelled with a fluorescent probe, DiI (Dil-LM), to examine the kinetics. Flow cytometric analysis demonstrated that in freshly isolated peripheral blood mononuclear cells (PBMC), monocytes principally took up Dil-LM, while lymphocytes and granulocytes did not. When PBMC were stimulated with immobilized anti-CD3 MoAb and IL-2, cells expressing CD3, CD4, CD8 and CD 16 incorporated Dil-LM. Purified CD4+ T cells, obtained by positive panning selection, were stimulated with this system. They were CD25, CD71, LFA-1-positive, and also showed an ability to take up Dil-LM, which resting cells did not. The findings were confirmed by flow cytometry and quantitative analysis of Dil. Confocal micrographs showed fluorescent granules from the probe in the cytoplasm of stimulated CD4+ T cells after incubation with DiI-LM. These results suggest that immunomodulatory agents incorporated into LM might selectively regulate the function of CD4+ or CD8+ T cells when these are activated.
Publisher
Oxford University Press (OUP)
Subject
Immunology,Immunology and Allergy
Reference31 articles.
1. Liposomes as drug carriers;Fendler;Life Sci,1977
2. Lipid composition is important for highly efficient target binding and retention of immunoliposomes;Maruyama;Proc Natl Acad Sci USA,1990
3. Tissue distribution and anti-inflammatory activity of corticosteroids incorporated in lipid emulsion;Mizushima;Ann Rheum Dis,1982
4. Lipid microspheres as novel drug carriers;Mizushima;Drugs Exp Clin Res,1985
5. Prostaglandin E1 incorporated in lipid microspheres (Lipo PGE1);Otomo;Drugs Exp Clin Res,1985
Cited by
6 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献