Differential effects of CD45 CD45R and CD45R0 monoclonal antibodies in modulating human B cell activation

Abstract

SUMMARY We have examined the effect of monoclonal antibodies (MoAbs) to different epitopes of the leucocyte common antigen (LCA). CD45, on anti-human immunoglobutin-primed B cell activation. Binding of MoAbs to restricted epitopes present on CD45 glycoproteins of 180 kD and 220 kD (designated CD45R0 and CD45R. respectively) was found to promote B cell proliferation in the presence of T cells. CD45 MoAbs reactive with ‘public’ determinants on all four constituent members of the LCA family (180, 190, 205, and 220 k D) had either little effect or inhibited the basal B cell response to anti-immunoglobulin priming. Simultaneous immunonuorcsccnt analysts of 5-broinodeoxyuridinc incorporation and the expression of CD19 (B cell specific) or CD2 (T cell specific) identified the majority of responder cells as B lymphocytes. CD45R MoAbs significantly enhanced the B cell response to sub-optimal concentrations of interleukin-2. CD45 and CD45R0 MoAbs failed to elicit a similar response. Antibody to the interleukin-2 receptor (anti-Tac) partially blocked the CD45R-driven. T cell-dependent B cell proliferation.