Low dose streptozotocin causes stimulation of the immune system and of anti-islet cytotoxicity in mice

Author:

KANTWERK-FUNKE G1,BURKART V1,KOLB H1

Affiliation:

1. Diabetes Research Institute, University of Düsseldorf, Düsseldorf, Germany

Abstract

SUMMARY Multiple low doses of streptozotocin arc known to induce immune-mediated insulin deficient diabetes and depression of immune reactivity. We show here that immune depression by streptozotocin is not general but that some parts of the immune system are stimulated. Spleen cells from streptozotocin-treated mice showed enhanced cytotoxicity against syngeneic islet cells and various tumour cells including insulinoma cells. Several cell types served as effector cells, including macrophages, asialo GM1+ and Lyt-2+ lymphocytes. The increased cytotoxic activity towards islet cells was mostly due to macrophages and to non-asialo GM1+ and non-Lyt-2+ lymphocytes. A higher activation state of macrophages in low dose streptozotocin-treated mice was demonstrated by measurements of superoxide anion release. We conclude that multiple low doses of streptozotocin stimulate ‘natural cytotoxicity’, i.e. the non-MHC restricted cytotoxic activity of macrophages, T cells and natural killer lymphocytes.

Publisher

Oxford University Press (OUP)

Subject

Immunology,Immunology and Allergy

Reference22 articles.

1. Spontaneous cytotoxicity of macrophages against pancreatic islet cells;Appels;J. Immunol,1989

2. Impairment of T-cell regulation of the humoral immune response to type III pneumococcal polysaccharide in diabetic mice;Busby;Diabetes,1983

3. Therapy with monoclonal antibodies by elimination of T-cell subsets in vivo;Cobbold;Nature,1984

4. Multiple low dose streptozotocin induces systemic MHC expression in mice by triggering T cells to release IFN-γ;Cockfield;J. Immunol,1989

5. Effect of helper and/or cytotoxic T-lymphocyte depletion on low-dose streptozocin-induced diabetes in C57B1/6J mice;Dayer-MetroZ;Diabetes,1988

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3